Virtual Labs

Biotechnology and Biomedical Engineering Molecular Biology Virtual Lab II Experiments

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

Polymerase Chain Reaction (PCR)

Choose difficulty:

Beginner

Intermediate

Advanced

The number of amplified pieces of DNA equals -------- after five cycles of PCR

a: 5 Explanation

Explanation

b: 10 Explanation

Explanation

c: 25 Explanation

Explanation

d: 32 Explanation

Explanation

Is it possible to use PCR to produce many copies of all DNA of one chromosome ?

a: yes, if the correct primers are available Explanation

Explanation

b: yes,if the telomeres are present Explanation

Explanation

c: No, PCR cannot copy DNA, only RNA Explanation

Explanation

d: No, PCR copies short pieces of DNA only. Explanation

Explanation

PCR requires knowledge about parts of a target DNA sequence to be amplified. True or False?

a: True Explanation

Explanation

b: False Explanation

Explanation

Which of the following is an advantage of single - primer PCR?

a: Less synthetic DNA is incorporated into the product Explanation

Explanation

b: Unknown sequence adjacent to a known sequence can be amplified with a single primer Explanation

Explanation

c: No Primer-dimer artifacts Explanation

Explanation

Which end of a PCR primer can be extended by polymerase?

a: The 1'end Explanation

Explanation

b: The 2'end Explanation

Explanation

c: The 3'end Explanation

Explanation

d: The 4'end Explanation

Explanation

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