Virtual Labs

Biotechnology and Biomedical Engineering Molecular Biology Virtual Lab II Experiments

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

Polyacrylamide Gel Electrophoresis

Choose difficulty:

Beginner

Intermediate

Advanced

In an SDS-PAGE gel

a: Proteins are denatured by SDS Explanation

Explanation

b: Proteins have the same charge to mass ratio Explanation

Explanation

c: Smaller proteins migrate more rapidly through the gel Explanation

Explanation

d: All the above Explanation

Explanation

Which of the following are considered part of the secondary structure of a protein?

a: Alpha helix Explanation

Explanation

b: Beta pleated sheet Explanation

Explanation

c: Disulfide bonds Explanation

Explanation

d: Both 1 and 2 Explanation

Explanation

SDS-PAGE has been used

a: To analyze different types of proteins in a biological sample Explanation

Explanation

b: To characterize membrane proteins in their native conformation Explanation

Explanation

c: To characterize the enzyme activities in the lysosome Explanation

Explanation

d: To isolate nuclear DNAs Explanation

Explanation

In an SDS-PAGE experiment, proteins are separated on the basis of their

a: Molecular weight Explanation

Explanation

b: Charge to mass ratio Explanation

Explanation

c: Different isoelectric points Explanation

Explanation

d: Negatively charged side chain Explanation

Explanation

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