Virtual Labs

T4 DNA ligase is similar to E. coli DNA ligase, except that ATP is a cofactor rather than NAD. Besides ligated DNA, another product is

a: pyrophosphate Explanation

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b: phosphate Explanation

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c: NMN Explanation

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d: NAD Explanation

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In order to produce a DNA fragment that can be inserted into the DNA of a second organism one would need to

a: find a virus that could serve as a vector for transfer of the donor DNA Explanation

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b: create a compatible DNA segment from an mRNA template Explanation

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c: cut the DNA of the donor and recipient cells with the same restriction enzyme Explanation

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d: be sure both donor and recipient DNA were prokaryotic or eukaryotic Explanation

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Cuts in DNA are sealed with

a: restriction enzymes Explanation

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b: reverse transcriptase Explanation

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c: polymerase Explanation

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d: ligases Explanation

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Sticky ends are created by

a: cutting DNA so that there are short, single strands at each end Explanation

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b: cutting the DNA fragment in several places Explanation

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c: adding polymerase to the end of the DNA fragment Explanation

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d: adding ligase to the end of the DNA fragment Explanation

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The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is

a: pharmacogenomics Explanation

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b: genetic engineering Explanation

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c: population genetics Explanation

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d: population dynamics Explanation

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Vectors are

a: molecules that are able to covalently bond to and carry foreign DNA into cells Explanation

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b: molecules that degrade nucleic acids Explanation

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c: molecules that protect host cells from invasion by foreign DNA Explanation

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d: both 1 & 2 Explanation

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Ligation ( Using T4 DNA Ligase)