Plasmid Curing

Objectives

Plasmids are extrachromasomally replicating molecules of DNA present in bacteria.Plasmid curing is a process of completely removing plasmids of bacteria by means of chemical agents such as Acriflavin ,acridine orange,SDS, etc. The objectives of the experiment include

  1. To obtain plasmid cured bacteria.
  2. To determine the effect of Acridine orange as plasmid curing agent.