Plasmid Curing
Objectives
Plasmids are extrachromasomally replicating molecules of DNA present in bacteria.Plasmid curing is a process of completely removing plasmids of bacteria by means of chemical agents such as Acriflavin ,acridine orange,SDS, etc. The objectives of the experiment include
- To obtain plasmid cured bacteria.
- To determine the effect of Acridine orange as plasmid curing agent.